[Purposes] After aging SBS （styrene-butadiene-styrene block copolymer） modified asphalt has some problems such as hardening embrittlement of matrix asphalt and degradation of SBS polymer. It is difficult to recover the properties of aging SBS modified asphalt effectively by traditional regeneration methods. In order to effectively restore the pavement performance of aged SBS asphalt, SBR （styrene-butadiene rubber） and bio-oil were used to regenerate the aged SBS modified asphalt.[Methods] Firstly, the aging SBS modified asphalt was prepared by indoor simulated aging test, and the composite recycled asphalt was obtained by adding SBR and bio-oil into the aging SBS modified asphalt by high-speed shear instrument; Then, the recycled asphalt was tested for three indexes and rheological properties at high and low temperatures; Finally, the mechanism of asphalt regeneration by SBR and bio-oil was investigated by infrared spectroscopy, atomic force microscope and fluorescence microscope. [Findings] The overall properties of recycled asphalt blended with SBR and bio-oil are better, and the complex shear modulus G* of recycled asphalt decreases with the increase of regenerant content. After the regeneration treatments, the recucled aphalts show reduced stiffness, restored flexibility, inproved stress dsspastion ability, decreased brittleness, and enhanced low temperature performance. The composite regeneration with SBR and bio-oil can supplement the light components of aged SBS modified asphalt. The ratio of asphaltene and colloid decreases after mixing SBR and bio-oil, and the light components can inhibit the wax crystallization of bee structure. The fluorescent substances of recycled asphalt increase greatly and distribute more densely, and the toughness and viscosity of aged SBS modified asphalt recover significantly. [Conclusions] The recommended content of SBR and bio-oil to restore the properties of aged SBS modified asphalt is 6% and 5%, respectively. This study provides a theoretical basis for the recycling of aged SBS modified asphalt mixture.