[Purposes]The paper aims to explore the effectiveness of the multi-scale algo- rithm in studying the mechanical characteristics of splitting fatigue damage of asphalt mix-ture．[Methods]By dividing five scale areas, the multi-scale analysis of five different gradations of asphalt mixtures was conducted, the splitting strengths were calculated under different fatigue damage degrees,the splitting strengths obtained by the indoor test and the multi-scale algorithm were compared and analyzed to verify the rationality of the multi-scale algorithm in studying the mechanical characteristics of splitting fatigue damage of asphalt mixture. [Findings]The splitting strength of asphalt mixture with aggregate particle size of 1.18-2.36 mm has been greatly improved, the absolute values of relative errors between the splitting strengths obtained by the multi-scale algorithm and that obtained by the indoor test are all within 10%, which meets the engineering accuracy requirements. [Conclusions]The multi-scale algorithm is feasible to study the mechanical characteristics of splitting fatigue damage of asphalt mixture, and has certain application value in practical engineering.