To explore the influence of various factors on the run-up of landslide generated waves in V-shaped valley, provide an effective and reliable basis for calculating the run-up value and assess the run-up hazard of landslide generated waves in V-shaped valley, the effects of several parameters, such as landslide volume, impact velocity, inserting angle, climbing angle and time of underwater sliding, on wave run-up were analyzed with data from 141 numerical simulation tests, and thus the empirical formula of run-up was proposed by the multiple regression analysis method. The new formula was applied to evaluate the wave run-up in Gongjiafang landslide event. It is shown from results that the new formula's calculated value is very close to the investigated value,which can provide a reference for calculating and predicting the run-up of landslide generated waves in other V-shaped valleys.