A novel fluorescence sensor was prepared via covalent immobilization of indicator dyes by using UV light polymerization technique. 1-Amino-4-allyloxyanthraquinone, a fluorescence indicator with olefin unit at the end of the molecule,was synthesized by reacting allyl bromide with 1-amino-4-hydroxyanthraquinone, followed by copolymerization with β- hydroxypropyl methacrylate and 1, 2-cyclohexanediol diacrylate on the surface-modified quartz glass slide under UV radiation. A 1-amino-4-allyloxyanthraquinone-immobilized fluorescence sensor was obtained. The sensor shows no dye-leaching, having a linear response toward ornidazole in the concentration range of 9.0 × 10^-6-1.0 × 10^-3 mol/L with a detection limit of 8 × 10^-6 mol/L,and recovery ratio is 96.7 %-103.9 %. It is simple and feasible for measuring ornidazole by the sensor. The response is rapid and the response time is within2 min.